After PH of starch milk regulated to 5.8,add enzyme in it and make temperature 70℃ sent it to water heating machine,increase the temperature instantaneously through heating machine and keep it warm in flow reactor,then complete liquefication with aid of enzyme.Flash the milk to 95℃ and add complementary enzyme, then enters flow reactor to keep warm,liquefied liquid will be ok if it turns to brown or red in iodine test.the qualified liquid`s temperature will fall down after heat transfer with low temperature starch milk,and becomes 63℃ through circulating water.add H2SO4 to regulate the PH value to 4.2 ,then add enzyme for saccharification ,check the DE value after 48 hours saccharification is finished when value reach above 98
Sacacharifized solution contains a few colloid,gluten,fiber need filtration ,it adopts automatic plate filter which have filtration by undercurrent that reduce pollution to environment of solution,sugar filtered is sent for feed while solution for concentration unit
Low concentration sugar syrup will be used as raw material, in the process fed-batch carbon source and nitrogen; control PH, dissolved oxygen and temperature to control the bacillus metabolic process and let the big amount of lysine accumulate until the conversion from sugar to acid is 65%, and acid production rate is above 17%.
The substrate material which is composed of corn steep liquid, molasses etc. enters fermentation tank as fermentation substrate material after continuous sterilization; thick syrup from concentration section and ammonium sulfate solution enters the fed-batch tank after heating sterilization by plate heat exchanger, and then enters each fermentation tank and seeding tanks as carbon source and nitrogen source;culture the qualified strain and connected to the fermenter through seeding tank,control the PH ,dissolved oxygen and temperature ,after about 50h fermentation culture ,when the acidity reaches about 17%,the fermentation process is finished and the overall transformation rate reaches 73%,then the fermentation liquor is sent into centrifuge for centrifugation separation after ph adjustment with adding H2SO4
3.65% lysine extraction workshop.
This workshop is concentrating the bottom flow of 98% lysine separator, then mix lysine mother liquor with other auxiliary material, all material is going to spraying, granulating and drying after they are mixed well, the 65% lysine sulfate.
Concentration section: raw material is bottom flow from separator with low DS content, first to be concentrated to 52.2%.
Blending section: blending the concentrated material and mother liquor from 98% lysine separation by proper ratio, then mix and blending with other auxiliary materials to adjust the lysine content.
Granulating, drying and packing section: To send the syrup to spray nozzle by granulating pump, then the syrup is atomized to mini drop of liquid then being sprayed on the fluid solid particals which grows bigger by agglomeration and coating function, the moisture is evaporated by hot air and internal heater, then dry granules are generated. The product goes packing into 25kg/bag from storage.
4.98% lysine extraction workshop.
98% lysine extraction workshop is composed of centrifuging separation, ISEP, concentration, neutralization crystallization, centrifuging drying and packing sections.
Fermentation liquid is adjusted then heated to 70-75℃ and then being pumped to the centrifuging separation section to have the filtrate.
After ISEP, Filtrate is purified and the waste liquid is sent to fertilizer workshop for granulation.
Purified liquid DS is about 19%（w/w）, enters ammonia-removing column after being heat exchange with vapor condensate from concentration section, then goes to evaporation and get 72%（w/w）DS concentrated liquid.
Concentrated liquid goes to neutralization section and mix with HCL to produce lysine hydrochloride, circulating water is used to cool down the mixed liquid for guarantee the stable crystallization.
Neutralized liquid mix with mother liquid and be sent to 1# crystallizer continuously, crystallization is going on continuously.
The crystal with liquid is separated as wet crystal which is sent to drying to have product; and part of mother liquid goes back with neutralization liquid and go on crystallization, another part goes to 65% lysine extraction workshop to produce lysine sulfate